Neurobiology of Disease In Vivo Inhibition of miR-155 Promotes Recovery after Experimental Mouse Stroke
نویسندگان
چکیده
Ernesto Caballero-Garrido,1 Juan Carlos Pena-Philippides,1 X Tamar Lordkipanidze,2 Denis Bragin,1 Yirong Yang,3 Erik Barry Erhardt,4 and X Tamara Roitbak1 1Department of Neurosurgery, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131, 2Ilia State University, 0162 Tbilisi, Georgia, and 3BRaIN Imaging Center and College of Pharmacy and 4Department of Mathematics and Statistics, University of New Mexico, Albuquerque, New Mexico 87131
منابع مشابه
In Vivo Inhibition of miR-155 Promotes Recovery after Experimental Mouse Stroke.
A multifunctional microRNA, miR-155, has been recently recognized as an important modulator of numerous biological processes. In our previous in vitro studies, miR-155 was identified as a potential regulator of the endothelial morphogenesis. The present study demonstrates that in vivo inhibition of miR-155 supports cerebral vasculature after experimental stroke. Intravenous injections of a spec...
متن کاملMicroRNA-155 promotes the pathogenesis of experimental colitis by repressing SHIP-1 expression
AIM To explore the mechanism by which microRNA-155 (miR-155) regulates the pathogenesis of experimental colitis. METHODS A luciferase assay was performed to confirm the binding of miR-155 to the SHIP-1 3'-UTR. MiR-155 mimics, negative controls and SHIP-1 expression/knockdown vectors were established and then utilized in gain- and loss-of-function studies performed in raw264.7 cells and primar...
متن کاملLoss of oncogenic miR-155 in tumor cells promotes tumor growth by enhancing C/EBP-β-mediated MDSC infiltration
The oncogenic role of microRNA-155 (miR-155) in leukemia is well established but its role in other cancers, especially breast cancer, is gradually emerging. In this study we examined the effect of mir-155 loss in a well-characterized spontaneous breast cancer mouse model where Brca1 and Trp53 are deleted by K14-Cre. miR-155 is known to be up-regulated in BRCA1-deficient tumors. Surprisingly, co...
متن کاملInfluence of miR-155 on Cell Apoptosis in Rats with Ischemic Stroke: Role of the Ras Homolog Enriched in Brain (Rheb)/mTOR Pathway
BACKGROUND We designed and carried out this study to examine the role of miR-155 and the Rheb/mTOR pathway in ischemic stroke. We also investigated how these two elements interact with each other and contribute to injuries resulting from ischemic stroke. MATERIAL AND METHODS We used both a middle cerebral artery occlusion rat model in vivo and an oxygen-glucose deprivation cell model in vitro t...
متن کاملSuppression of miR-155 Expression in IFN-γ-Treated Astrocytes and Microglia by DJ-1: A Possible Mechanism for Maintaining SOCS1 Expression
Previously, we reported that DJ-1, encoded by a Parkinson's disease (PD)-associated gene, inhibits expression of proinflammatory mediators in interferon-gamma (IFN-γ)-treated astrocytes and microglia through inhibition of STAT1 activation. Here, using microglia and astrocytes cultured from wild-type (WT) and DJ-1-knockout (KO) mouse brains, we examined how DJ-1 regulates suppressor of cytokine ...
متن کامل